Here is a summary of the info I gleaned from asking for recommendations for the best human cell line. These were my criteria: For context, we currently use a number of human cell lines in the lab: HeLa, RPE1, HCT116, SKOV3; as well as many others in the past: HEK293, DLD-1, U2OS. I consider HeLa to be the almost perfect cell line.

Quantifying the degree of colocalisation of two signals in microscopy images is very tricky. Lots has been written on this topic, including in my book The Digital Cell. The focus of this post is on visualising colocalisation . One way to look at colocalisation is two think about two sets of objects and how many of each set overlap. This is sometimes referred to as co-occupancy or object-based colocalisation .

A new paper means a new paper explainer. This post is all about our new paper on clathrin assembly. Some background info Endocytosis is the way that cells take up material from the outside world. The cell can make tiny vesicles that bud inwards from the cell surface and pinch off to travel inside the cell. This process is important for lots of things that cells do, so there is a lot of interest in how endocytosis works.

I wrote a short opinion piece for the December Newsletter for the American Society for Cell Biology (ASCB). The content is reproduced below, or you can read the newsletter version here on page 14 of the PDF. The theme of this year’s ASCB|EMBO Meeting is Cell Biology for the 21st Century. So what skills are essential for a cell biologist to master in the 21st century?

We have a new paper out! You can access it here. The paper in a nutshell We have discovered a new class of trafficking vesicle inside cells. These vesicles are very small (30 nm across) and we’ve called them intracellular nanovesicles, or INVs for short. What is a trafficking vesicle? Humans are built from lots of cells.

This wonderful movie has repeatedly popped up into my twitter feed. http://quantixed.org/wp-content/uploads/2018/04/ctenophore.mp4 It was taken by Tessa Montague and is available here (tweet is here). The movie is striking because of the way that cytokinesis starts at one side and moves to the other. Most model systems for cell division have symmetrical division. Rob de Bruin commented that “it makes total sense to segregate this way”.

We have a new paper out. The title is New tools for ‘hot-wiring’ clathrin-mediated endocytosis with temporal and spatial precision . You can read it here. Cells have a plasma membrane which is the barrier between the cell’s interior and the outside world. In order to import material from outside, cells have a special process called endocytosis.

We have a new paper out! This post is to explain what it’s about. Cancer cells often have gene fusions . This happens because the DNA in cancer cells is really messed up. Sometimes, chromosomes can break and get reattached to a different one in a strange way. This means you get a fusion between one gene and another which makes a new gene, called a gene fusion.

We have a new paper out! You can access it here. The people This paper really was a team effort. Faye Nixon and Tom Honnor are joint-first authors. Faye did most of the experimental work in the final months of her PhD and Tom came up with the idea for the mathematical modelling and helped to rewrite our analysis method in R. Other people helped in lots of ways.

I’ve returned from the American Society for Cell Biology 2016 meeting in San Francisco. Despite being a cell biologist and people from my lab attending this meeting numerous times, this was my first ASCB meeting. The conference was amazing, so much excellent science and so many opportunities to meet up with people.