Euan Adie has asked for some help to do further analysis on the comments made on PLoS ONE articles. He is doing this via crowd sourcing through a specially written app at appspot to get people to characterize all the comments in PLoS ONE. Euan is very good at putting these kind of things together and again this shows the power of Friendfeed as a way of getting the message out.

…but being straightforward is always the best approach. Since we published our paper in PLoS ONE a few months back I haven’t been as happy as I was about the activity of our Sortase. What this means is that we are now using a higher concentration of the enzyme to do our ligation reactions. They seem to be working well and with high yields, but we need to put in more enzyme.

This post is an opinion piece and not a rigorous objective analysis. It is fair to say that I am on the record as and advocate of the principles behind PLoS ONE and am also in favour of post publication peer review and this should be read in that light. [ ed I’ve also modified this slightly from the original version because I got myself mixed up in an Excel spreadsheet] To me, anonymous peer review is, and always has been, broken.